Application of a rapid gel method to the sequencing of fragments of 16S ribosomal RNA from Escherichia coil | Zendy

Alexander Ross | Zendy; B Halberg Richard | Zendy

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Application of a rapid gel method to the sequencing of fragments of 16S ribosomal RNA from Escherichia coil

Author(s) -

Alexander Ross,

B Halberg Richard

Publication year - 1978

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/5.1.241

Subject(s) - biology , ribonuclease t1 , rna , 16s ribosomal rna , oligonucleotide , ribosomal rna , guanine , microbiology and biotechnology , ribosome , 5.8s ribosomal rna , nucleotide , biochemistry , ribonuclease , nucleic acid sequence , genetics , dna , rnase p , gene

A gel sequencing method has been applied to two 5' end-labelled fragments of the 16S ribosomal RNA from E. coli. The procedure involves partial enzymatic hydrolysis by ribonucleases T1, U2 or A, in order to generate series of end-labelled subfragments terminating in guanine, adenine, or pyrimidine residues, respectively. The two fragments concerned were approximately 75 and 90 nucleotides in length, and both arose from the 3' region of the 16S RNA. The sequences deduced are compared with the published sequence of 16S RNA, and contribute information to the final ordering of the ribonuclease T1 oligonucleotides in the latter, as well as revealing some probable errors.

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