Open AccessLac repressor purification without inactivation of DNA binding activity
Author(s) -
John M. Rosenberg,
Olga B. Khallai,
Mary L. Kopka,
Richard E. Dickerson,
Arthur D. Riggs
Publication year - 1977
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/4.3.567
Subject(s) - repressor , biology , dna , lac repressor , operator (biology) , dna binding protein , microbiology and biotechnology , biochemistry , gene , transcription factor
A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 +/- 10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA.
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