Fluoresceinylthiocarbarnyl-tRNATyr: a useful derivative of tRNATyr(E.coli) for physico-chemical studies | Zendy

Alfred Pingoud | Zendy; R. Kownatzki | Zendy; Guenter Maass | Zendy

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Fluoresceinylthiocarbarnyl-tRNA^Tyr: a useful derivative of tRNA^Tyr(E.coli) for physico-chemical studies

Author(s) -

Alfred Pingoud,

R. Kownatzki,

Guenter Maass

Publication year - 1977

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/4.2.327

Subject(s) - transfer rna , ef tu , biology , aminoacylation , biochemistry , rna , elongation factor , amino acyl trna synthetases , ribosome , gene

Fluoresceinylthiocarbamyl-tRNATyr (FTC-tRNATyr) is prepared from tRNATyr and fluoresceinisothiogyanate (FITC) under mildly alkaline conditions. Labelling occurs specificly at the base Q of tRNATyr. The modified tRNA is fully active in the aminoacylation assay; when aminoacylated it is recognized by the elongation factor Tu (EF-Tu). Codon-anticodon interaction, however, is severely affected by the modification.

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