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Fluoresceinylthiocarbamyl-tRNATyr (FTC-tRNATyr) is prepared from tRNATyr and fluoresceinisothiogyanate (FITC) under mildly alkaline conditions. Labelling occurs specificly at the base Q of tRNATyr. The modified tRNA is fully active in the aminoacylation assay; when aminoacylated it is recognized by the elongation factor Tu (EF-Tu). Codon-anticodon interaction, however, is severely affected by the modification.

Fluoresceinylthiocarbarnyl-tRNATyr: a useful derivative of tRNATyr(E.coli) for physico-chemical studies

Fluoresceinylthiocarbarnyl-tRNA^Tyr: a useful derivative of tRNA^Tyr(E.coli) for physico-chemical studies