Open AccessThe SV4O transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity
Author(s) -
Timothy L. Brooks,
Melvin H. Green
Publication year - 1977
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/4.12.4261
Subject(s) - biology , rna polymerase ii , transcription (linguistics) , chromatin , rna polymerase , polymerase , endogeny , nucleic acid , rna , microbiology and biotechnology , genetics , dna , gene , biochemistry , gene expression , promoter , philosophy , linguistics
SV40 chromatin obtained from infected monkey cells was used to study the effect of the viral chromatin proteins on endogenous RNA polymerase II. Ammonium sulfate activated the rate of transcription by endogenous RNA polymerase in two ways: 1) by direct action on the enzyme; and 2) by causing a reversible conformational change in the viral chromatin. Under optimal reaction conditions, the viral chromatin proteins did not limit the rate of RNA chain elongation, and high molecular weight RNA (1.6 X 10(6) d) was synthesized by the SV40 chromatin.