Heterogeneity of 5′-termini of nucleolar 45S, 32S and 28S RNA In mouse hepatoma | Zendy

Ryo Kominami | Zendy; Maasami Muramatsu | Zendy

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Heterogeneity of 5′-termini of nucleolar 45S, 32S and 28S RNA In mouse hepatoma

Author(s) -

Ryo Kominami,

Maasami Muramatsu

Publication year - 1977

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/4.1.229

Subject(s) - biology , rna , 28s ribosomal rna , ribosomal rna , cleavage (geology) , microbiology and biotechnology , gene , transcription (linguistics) , biochemistry , genetics , ribosome , paleontology , fracture (geology) , linguistics , philosophy

The 5'-termini of nucleolar 45S, 32S and 28S RNA's were analyzed by means of thin layer chromatography and Dowex-1 colum chromatography. 45S RNA did not bear a triphosphate at the 5'-terminus, but various monophosphates are found. 5'-termini of 32S and 28S RNA's were also heterogeneous. These results indicate that 45S molecules as isolated with the conventional procedure may not contains the primary transcript of the ribosomal gene, but a collection of large precursors with different degrees of processing at the 5'-terminus. The processing of the primary transcript may thus involve some unknown trimming processes at the 5'-terminus before the first major cleavage takes place.

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