Open AccessChemical incorporation of 1-methyladenosine into oligonucleotides
Author(s) -
Sergey N. Mikhailov
Publication year - 2002
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/30.5.1124
Subject(s) - oligonucleotide , biology , moiety , nucleoside , dna , rna , biochemistry , anhydrous , n6 methyladenosine , nucleotide , nucleic acid , combinatorial chemistry , stereochemistry , methyltransferase , gene , organic chemistry , chemistry , methylation
The base moiety of 1-N-methyladenosine can be protected with a chloroacetyl group for incorporation of this modified nucleoside into DNA and RNA. Carefully controlled anhydrous conditions are needed for deprotection of the oligonucleotides.
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