Elimination of double strand nuclease activity from S1 nuclease prepared from crude amylase | Zendy

William E. Hahn | Zendy; J Van Ness | Zendy

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Elimination of double strand nuclease activity from S1 nuclease prepared from crude amylase

Author(s) -

William E. Hahn,

J Van Ness

Publication year - 1976

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/3.5.1419

Subject(s) - nuclease , biology , dna , nucleic acid , biochemistry , endonuclease , rna , micrococcal nuclease , double strand , microbiology and biotechnology , dna repair , gene , nucleosome , histone

Single strand-specific s1 nuclease prepared as previously described from crude alpha amylase by DEAE-cellulose chromatography also contains nuclease which degrades double strand nucleic acid. The double strand activity can be removed by repeating the DEAE-cellulose chromatography procedure at least two additional times. S1 nuclease prepared by this procedure does not degrade double strand sheared DNA as measured by Sephadex chromatography. Under the same conditions single strand DNA is completely degraded. Thus, S1 nuclease prepared by this procedure is suitable for use in removing single strand regions in DNA/DNA duplexes and DNA/RNA hybrids.

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