Isolation of single stranded DNA related to the transcriptional activity of animal cells | Zendy

Haïm Tapiero | Zendy; S.A. Leibowitch | Zendy; D. Shaool | Zendy; M.N. Monier | Zendy; J Harel | Zendy

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Isolation of single stranded DNA related to the transcriptional activity of animal cells

Author(s) -

Haïm Tapiero,

S.A. Leibowitch,

D. Shaool,

M.N. Monier,

J Harel

Publication year - 1976

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/3.4.953

Subject(s) - biology , dna , microbiology and biotechnology , rna , transcription (linguistics) , nuclease , nuclear dna , nucleic acid thermodynamics , thymidine , genome , biochemistry , gene , mitochondrial dna , base sequence , philosophy , linguistics

Single stranded DNA (s.s.DNA) comprising 1-2% of the total nuclear DNA was isolated by an improved method of hydroxyapatite chromatography from native nuclear DNA3 of embryonic chick cells, labeled for several cell generations with 3H-thymidine. Small quantities of 3H-DNA were annealed with a large excess of unlabeled DNA or polysomal RNA from chick embryos. Hybridization kinetics (monitored by the use of SI nuclease digestion, hydroxyapatite chromatography and thermalfusion), indicated that s.s.DNA belongs to the non repetitious fraction of the cell genome. One third represents DNA sequences engaged in the transcription of messenger RNA's.

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