The methylation of adenovirus-specific nuclear and cytoplasmic RNA | Zendy

Stefanie Sommer | Zendy; M. Salditt-Georgieff | Zendy; Steven L. Bachenheimer | Zendy; James Darnell | Zendy; Yasuhiro Furuichi | Zendy; M. Morgan | Zendy; Aaron J. Shatkin | Zendy

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The methylation of adenovirus-specific nuclear and cytoplasmic RNA

Author(s) -

Stefanie Sommer,

M. Salditt-Georgieff,

Steven L. Bachenheimer,

James Darnell,

Yasuhiro Furuichi,

M. Morgan,

Aaron J. Shatkin

Publication year - 1976

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/3.3.749

Subject(s) - biology , methylation , rna , cytoplasm , small nuclear rna , dna methylation , cell nucleus , microbiology and biotechnology , virology , genetics , dna , non coding rna , gene expression , gene

Each poly(A) containing cytoplasmic AD-2 MRNA contains at its 5' terminus the general structure m7 GpppN1 pN2p or m7 GpppN1mpN2mpNp as well as an average of 4 m6A and 0.5-1 m5C residues per molecule. Almost all of the N1m residues are adenine derivatives including Am, m6Am and probably m26,6Am. The N2m is mostly Cm but small amounts of the other three methylated bases are also present. All the methylated constitutents of mRNA are distant from the 3' terminal poly(A). The amount of m6A appears to be greater in larger mRNA than in smaller mRNA. Nuclear Ad-2 specific RNA also contains caps, m6A, and m5C with about twice as much m6A relative to caps as cytoplasmic mRNA. The similarity of Ad-2 nuclear and mRNA to HeLa hnRNA and mRNA suggests that adenovirus mRNA production is a good model for eukaryotic mRNA production.

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