Nucleotide sequence of the restriction fragments Hind L and Hind M of SV40 DNA | Zendy

M. Ysebaert | Zendy; F. Thys | Zendy; A. Van de Voorde | Zendy; Walter Fiers | Zendy

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Nucleotide sequence of the restriction fragments Hind L and Hind M of SV40 DNA

Author(s) -

M. Ysebaert,

F. Thys,

A. Van de Voorde,

Walter Fiers

Publication year - 1976

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/3.12.3409

Subject(s) - biology , dna , hindiii , microbiology and biotechnology , nucleic acid sequence , restriction site , sequence (biology) , nucleotide , restriction fragment , hindlimb , restriction map , stop codon , base pair , restriction enzyme , genetics , gene , anatomy

The nucleotide sequence of the two minor SV40 DNA restriction fragments Hind L and Hind M is here reported. For this purpose we used 5'-32p-labeled DNA fragments either partially digested with snake venom diesterase for analysis by the wandering spot method or partially degraded with the base specific reagents dimethylsulphate or hydrazine for direct sequence analysis on gel. In the former procedure the strands were separated before degradation, while in the latter the strands were separated after modification but before the degradation. Due to the presence of several termination codons, the region Hind L - Hind M cannot be translated in a polypeptide. Also, no initiation codons for protein synthesis are present in this region.

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