A possible structure for calf satellite DNA I | Zendy

Gérard Roizès | Zendy

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A possible structure for calf satellite DNA I

Author(s) -

Gérard Roizès

Publication year - 1976

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/3.10.2677

Subject(s) - biology , restriction enzyme , satellite dna , ecori , satellite , dna , cpg site , restriction site , microbiology and biotechnology , hindiii , genetics , restriction fragment , restriction map , endonuclease , nucleic acid sequence , gene , genome , dna methylation , gene expression , engineering , aerospace engineering

Calf satellite DNA I (p = 1.715) has been hydrolysed by a number or restriction endonucleases. It consists of a repeating unit of 1460 nucleotide pairs within which the sites of Eco R II Mbo I, Sac I, Alu I, Ava II and Hha I were localised in comparison with those of Eco R I and Hind II. The distribution of the Hpa II, Sac I, Hha I, Hinf I and Mbo II sites within calf satellite DNA I, as well as that of several restriction endonuclease sites within calf satellite DNA III (p = 1.705) allowed me to define subsatellite fractions. Furthermore, some of the sites of the CpG containing restriction enzymes Hpa II and Hha I are lacking. The possible implications of these results are discussed.

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