Open AccessDuplication of single stranded DNA catalyzed by calf thymus DNA polymerase
Author(s) -
Er-Chung Wang,
David Henner,
J. Fürth
Publication year - 1976
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/3.1.129
Subject(s) - biology , dna clamp , dna polymerase , dna polymerase ii , microbiology and biotechnology , dna , nuclease , dna polymerase i , dna replication , polymerase , biochemistry , polymerase chain reaction , gene , reverse transcriptase
Purified calf thymus DNA polymerase alpha is inactive with native DNA as template and shows little activity with denatured DNA. DNA synthesis with denatured DNA as template is greatly stimulated by the addition of a nuclease which initially copurifies with DNA polymerase but is separated from the polymerase on DEAE-cellulose chromatography. A limit digest of nuclease treated native DNA which is then denatured is replicated 80-95%; extensive replication is also obtained with native DNA partially degraded by pancreatic DNase and then denatured. The product of the reaction with calf thymus nuclease-treated DNA as template is double-stranded DNA with a hairpin (looped back) structure.
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