Open AccessTranscriptional activation by hepatocyte nuclear factor-4 in a cell-free system derived from rat liver nuclei
Author(s) -
S. K. Harish,
Tasneem Khanam,
Sendurai A. Mani,
Pundi N. Rangarajan
Publication year - 2001
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/29.5.1047
Subject(s) - transactivation , biology , hepatocyte nuclear factor 4 , transcription factor , microbiology and biotechnology , hepatocyte nuclear factors , nuclear protein , promoter , binding site , dna binding protein , transcription (linguistics) , basic helix loop helix leucine zipper transcription factors , gene expression , gene , biochemistry , nuclear receptor , linguistics , philosophy
Hepatocyte nuclear factor-4 (HNF4) regulates gene expression by binding to direct repeat motifs of the RG(G/T)TCA sequence separated by one nucleotide (DR1). In this study we demonstrate that endogenous HNF4 present in rat liver nuclear extracts, as well as purified recombinant HNF4, activates transcription from naked DNA templates containing multiple copies of the DR1 element linked to the adenovirus major late promoter. Recombinant HNF4 also activates transcription from the rat cellular retinol binding protein II (CRBPII) promoter in vitro. The region between -105 and -63 bp of this promoter is essential for HNF-mediated transactivation. The addition of a peptide containing the LXXLL motif abolished HNF4-mediated transactivation in vitro suggesting that LXXLL-containing protein factor(s) are involved in HNF4-mediated transactivation in rat liver nuclear extracts. This is the first report on transactivation by HNF4 in a cell-free system derived from rat liver nuclei.
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