Arrayed transposase-binding sequences on the ends of transposon Tn5090/Tn402 | Zendy

Masood KamaliMoghaddam | Zendy

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Arrayed transposase-binding sequences on the ends of transposon Tn5090/Tn402

Author(s) -

Masood KamaliMoghaddam

Publication year - 2001

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/29.4.1005

Subject(s) - transposase , biology , transposable element , inverted repeat , dna , genetics , cleavage (geology) , binding site , dna binding site , dna binding protein , peptide sequence , tandem repeat , microbiology and biotechnology , gene , transcription factor , promoter , gene expression , genome , paleontology , fracture (geology)

The transposon Tn5090/Tn402 encodes a 559 amino acid transposase, TniA, with a DDE motif. Gel mobility shifting and cleavage protection analysis with DNase I and hydroxyl radical probes revealed that TniA binds to multiple repeat sequences on either terminus of Tn5090/Tn402. Four of these TniA-binding 19mers occurred on the left-hand (t) end and two on the right-hand (i) end. Hydroxyl radical cleavage protection demonstrated the presence of 3-6 bp contact sequences on one face of the DNA helix. The binding pattern and organisation of repeats suggested parallels between Tn5090/Tn402 and Mu, which controls its transpositional activity in the assembly step of a higher order transpososome complex. The complex terminal structure and genes of transposase and nucleotide-binding proteins in tandem are hallmarks of the handful of Mu-like elements that are known to date.

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