RNase H mediated cleavage of RNA by cyclohexene nucleic acid (CeNA) | Zendy

Birgit Verbeure | Zendy

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RNase H mediated cleavage of RNA by cyclohexene nucleic acid (CeNA)

Author(s) -

Birgit Verbeure

Publication year - 2001

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/29.24.4941

Subject(s) - rna , nucleic acid , rnase h , dna , rnase p , duplex (building) , cleavage (geology) , ribonuclease , biology , biochemistry , nucleotide , nucleic acid structure , microbiology and biotechnology , gene , paleontology , fracture (geology)

Cyclohexene nucleic acid (CeNA) forms a duplex with RNA that is more stable than a DNA-RNA duplex (DeltaTm per modification: +2 degrees C). A cyclohexenyl A nucleotide adopts a 3'-endo conformation when introduced in dsDNA. The neighbouring deoxynucleotide adopts an O4'-endo conformation. The CeNA:RNA duplex is cleaved by RNase H. The Vmax and Km of the cleavage reaction for CeNA:RNA and DNA:RNA is in the same range, although the kcat value is about 600 times lower in the case of CeNA:RNA.

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