Preparing a human membrane and secreted protein-enriched cDNA library using PCR primers derived from a genomic database | Zendy

Yong Fan | Zendy

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Preparing a human membrane and secreted protein-enriched cDNA library using PCR primers derived from a genomic database

Author(s) -

Yong Fan

Publication year - 2001

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/29.22.e114

Subject(s) - biology , complementary dna , genomic library , polymerase chain reaction , cdna library , genomic dna , database , genetics , microbiology and biotechnology , gene , computational biology , base sequence , computer science

We describe here a strategy for preparing a human membrane and secreted protein (MSP)-enriched cDNA library based on human MSP- and non-MSP-encoding cDNA sequences in the databases. The signal peptide parts of the MSP-encoding cDNA sequences, which currently comprise about half of the estimated total number in humans, were analyzed for common patterns. These patterns form a 'minimal' set of polymerase chain reaction primer candidates of length varying from 9 to 21 nt. The products stemming from each primer candidate were determined and the results allowed us to obtain an 'optimal' mixed-length primer set. Ninety-six percent of the primers in this set were predicted to yield

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