An mRNA degrading complex in Rhodobacter capsulatus | Zendy

S. Jäger | Zendy

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An mRNA degrading complex in Rhodobacter capsulatus

Author(s) -

S. Jäger

Publication year - 2001

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/29.22.4581

Subject(s) - rhodobacter , biology , degradosome , rna helicase a , rnase p , helicase , rna , biochemistry , microbiology and biotechnology , gene , mutant

An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation, and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.

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