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Monolayers of single-stranded DNA on gold substrates were studied by scanning force microscopy. Complementary DNA probes labeled by gold nanoparticles were applied for contrast enhancement. Substrate regions modified with DNA could be visualized in a highly specific manner. The influence of the solution concentration on the surface density of adsorbed nanoparticles could be visualized. Because individual label particles can be easily detected, this labeling technique opens the way for characterization of DNA monolayers with a lateral resolution in the nanometer range.

DNA monolayer on gold substrates characterized by nanoparticle labeling and scanning force microscopy