Novel display of knotted DNA molecules by two-dimensional gel electrophoresis | Zendy

Sònia Trigueros | Zendy

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Novel display of knotted DNA molecules by two-dimensional gel electrophoresis

Author(s) -

Sònia Trigueros

Publication year - 2001

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/29.13.e67

Subject(s) - biology , dna , gel electrophoresis , agarose gel electrophoresis , electrophoresis , gel electrophoresis of nucleic acids , agarose , microbiology and biotechnology , genetics

We describe a two-dimensional agarose gel electrophoresis procedure that improves the resolution of knotted DNA molecules. The first gel dimension is run at low voltage, and DNA knots migrate according to their compactness. The second gel dimension is run at high voltage, and DNA knots migrate according to other physical parameters such as shape and flexibility. In comparison with one-dimensional gel electrophoresis, this procedure segregates the knotted DNA molecules from other unknotted forms of DNA, and partially resolves populations of knots that have the same number of crossings. The two-dimensional display may allow quantitative and qualitative characterization of different types of DNA knots simply by gel velocity.

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