Octamer-primed sequencing technology: effects of dNTP | Zendy

Anelia Kraltcheva | Zendy; Susan H. Hardin | Zendy

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Octamer-primed sequencing technology: effects of dNTP

Author(s) -

Anelia Kraltcheva,

Susan H. Hardin

Publication year - 2001

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/29.10.e48

Subject(s) - histone octamer , biology , primer (cosmetics) , dna sequencing , genomic sequencing , sequencing by ligation , genetics , sequence (biology) , dna , computational biology , genomic library , base sequence , gene , genome , histone , chemistry , nucleosome , organic chemistry

Octamer-primed sequencing is a directed DNA sequencing strategy that employs the use of a presynthesized octamer primer library. Together with electronic octamer sequencing technology (eOST) it provides a faster, less expensive way to obtain DNA sequence information and can be used as a valuable tool for gap closure in large-scale genomic sequencing. In this paper we discuss the effect of dGTP/TTP supplementation on octamer sequencing. We show that addition of 75 µ Md GTP and 5µM TTP can improve the sequencing success rate by increasing the length and accuracy of generated sequence information. We also discuss the effect of template base composition immediately downstream of the octamer primer on the outcome of octamer sequencing.

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