A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis | Zendy

Matthew C. Biery | Zendy

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A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis

Author(s) -

Matthew C. Biery

Publication year - 2000

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/28.5.1067

Subject(s) - biology , transposable element , cosmid , gene , plasmid , genetics , genome , transposition (logic) , transposase , dna , linguistics , philosophy

A robust Tn7-based in vitro transposition system is described that displays little target site selectivity, allowing the efficient recovery of many different transposon insertions in target DNAs ranging from small plasmids to cosmids to whole genomes. Two miniTn7 derivatives are described that are useful for the analysis of genes: one a derivative for making translational and transcriptional target gene fusions and the other a derivative that can generate 15 bp (5 amino acid) insertions in target DNAs (proteins).

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