Conformational transition in DNA on a cold surface | Zendy

Xi-Z. Feng | Zendy

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Conformational transition in DNA on a cold surface

Author(s) -

Xi-Z. Feng

Publication year - 2000

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/28.2.593

Subject(s) - biology , dna , biophysics , mica , molecule , crystallography , biochemistry , chemistry , paleontology , organic chemistry

The contour length of DNA fragments, deposited and imaged on mica under buffer, was measured as a function of deposition temperature. Extended DNA molecules (on Ni- and silane-treated surfaces) contract rapidly with falling temperature, approaching the contour length of A-DNA at 2 degrees C. The contraction is not unique to a specific sequence and does not occur in solution at 2 degrees C or on a surface at 25 degrees C, indicating that it arises from a combination of low temperature and surface contact. It is probably a consequence of reduced water activity at a cold surface.

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