Identification of sequence motifs in oligonucleotides whose presence is correlated with antisense activity | Zendy

O. V. Matveeva | Zendy; Alexander Tsodikov | Zendy; Michael C. Giddings | Zendy; Susan M. Freier | Zendy; Jacqueline R. Wyatt | Zendy; Alexey N. Spiridonov | Zendy; Svetlana A. Shabalina | Zendy; R.F. Gesteland | Zendy; John F. Atkins | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Identification of sequence motifs in oligonucleotides whose presence is correlated with antisense activity

Author(s) -

O. V. Matveeva,

Alexander Tsodikov,

Michael C. Giddings,

Susan M. Freier,

Jacqueline R. Wyatt,

Alexey N. Spiridonov,

Svetlana A. Shabalina,

R.F. Gesteland,

John F. Atkins

Publication year - 2000

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/28.15.2862

Subject(s) - oligonucleotide , biology , sequence motif , microbiology and biotechnology , messenger rna , sequence (biology) , computational biology , genetics , gene

Design of antisense oligonucleotides targeting any mRNA can be much more efficient when several activity-enhancing motifs are included and activity-decreasing motifs are avoided. This conclusion was made after statistical analysis of data collected from >1000 experiments with phosphorothioate-modified oligonucleotides. Highly significant positive correlation between the presence of motifs CCAC, TCCC, ACTC, GCCA and CTCT in the oligonucleotide and its antisense efficiency was demonstrated. In addition, negative correlation was revealed for the motifs GGGG, ACTG, AAA and TAA. It was found that the likelihood of activity of an oligonucleotide against a desired mRNA target is sequence motif content dependent.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research