Isolation of an essential Schizosaccharomyces pombe gene, prp31+, that links splicing and meiosis | Zendy

D. Timothy Bishop | Zendy

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Isolation of an essential Schizosaccharomyces pombe gene, prp31+, that links splicing and meiosis

Author(s) -

D. Timothy Bishop

Publication year - 2000

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/28.11.2214

Subject(s) - biology , schizosaccharomyces pombe , mutant , genetics , complementation , schizosaccharomyces , rna splicing , gene , mutation , meiosis , allele , homologous recombination , splicing factor , microbiology and biotechnology , rna

We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31(+) gene. The prp31-E1 mutant is defective in vegetative cell growth and in meiotic progression. It is synthetically lethal with prp6 and displays a pre-mRNA splicing defect at the restrictive temperature. We cloned the wild-type gene by complementation of the temperature-sensitive mutant phenotype. Prp31p is closely related to human and budding yeast PRP31 homologs and is likely to function as a general splicing factor in both vegetative growth and sexual differentiation.

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