DNA repair in a yeast origin of replication: contributions of photolyase and nucleotide excision repair | Zendy

Bernhard Suter | Zendy

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DNA repair in a yeast origin of replication: contributions of photolyase and nucleotide excision repair

Author(s) -

Bernhard Suter

Publication year - 2000

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/28.10.2060

Subject(s) - photolyase , biology , nucleotide excision repair , pyrimidine dimer , dna repair , chromatin , dna replication , replication protein a , genetics , dna damage , dna , nucleosome , nuclease , microbiology and biotechnology , dna binding protein , gene , transcription factor

DNA damage formation and repair are tightly linked to protein-DNA interactions in chromatin. We have used minichromosomes in yeast as chromatin substrates in vivo to investigate how nucleotide excision repair (NER) and repair by DNA-photolyase (photoreactivation) remove pyrimidine dimers from an origin of replication ( ARS1 ). The ARS1 region is nuclease sensitive and flanked by nucleosomes on both sides. Photoreactivation was generally faster than NER at all sites. Site-specific heterogeneity of repair was observed for both pathways. This heterogeneity was different for NER and photoreactivation and it was altered in a minichromosome where ARS1 was transcribed. The results indicate distinct inter-actions of the repair systems with protein complexes bound in the ARS region (ORC, Abf1) and a predominant role of photolyase in CPD repair of an origin of replication.

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