Open AccessHuman DNA topoisomerase I-mediated cleavage and recombination of duck hepatitis B virus DNA in vitro
Author(s) -
Philippe Pourquier,
Yves Pommier,
Alexandra D Jensen,
Shuran Gong,
C. E. Rogler
Publication year - 1999
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/27.8.1919
Subject(s) - biology , duck hepatitis b virus , dna , in vitro recombination , microbiology and biotechnology , dna supercoil , nuclease , topoisomerase , dna polymerase ii , oligonucleotide , replication protein a , dna clamp , dna replication , hepatitis b virus , virology , hepadnaviridae , genetics , virus , reverse transcriptase , gene , molecular cloning , dna binding protein , polymerase chain reaction , peptide sequence , transcription factor
In this study, we report that eukaryotic topoisomerase I (top1) can linearize the open circular DNA of duck hepatitis B virus (DHBV). Using synthetic oligonucleotides mimicking the three-strand flap DR1 region of the DHBV genome, we found that top1 cleaves the DNA plus strand in a suicidal manner, which mimics the linearization of the virion DNA. We also report that top1 can cleave the DNA minus strand at specific sites and can linearize the minus strand via a non-homologous recombination reaction. These results are consistent with the possibility that top1 can act as a DNA endo-nuclease and strand transferase and play a role in the circularization, linearization and possibly integration of viral replication intermediates.
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