Rapid modification of bacterial artificial chromosomes by ET- recombination | Zendy

Joep P. P. Muyrers | Zendy

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Rapid modification of bacterial artificial chromosomes by ET- recombination

Author(s) -

Joep P. P. Muyrers

Publication year - 1999

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/27.6.1555

Subject(s) - biology , homologous recombination , recombination , genetics , bacterial artificial chromosome , homology (biology) , cloning (programming) , flp frt recombination , computational biology , microbiology and biotechnology , dna , genetic recombination , gene , genome , computer science , programming language

We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable to any BAC modification desired.

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