All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature | Zendy

Thorsten Bechert | Zendy; Susanne Heck | Zendy; Ursula Fleig | Zendy; Stephan Diekmann | Zendy; Johannes H. Hegemann | Zendy

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All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature

Author(s) -

Thorsten Bechert,

Susanne Heck,

Ursula Fleig,

Stephan Diekmann,

Johannes H. Hegemann

Publication year - 1999

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/27.6.1444

Subject(s) - centromere , biology , saccharomyces cerevisiae , dna , genetics , repeated sequence , microbiology and biotechnology , yeast , chromosome , genome , gene

All 16 centromere DNA regions of Saccharomyces cerevisiae including 90 bp framing sequences on either side were cloned. These 300 bp long centromere regions were analysed by native polyacrylamide gel electrophoresis and found to display a reduced mobility indicative of DNA curvature. The degree of curvature is centromere dependent. The experimental data were confirmed by computer analysis of the 3-dimensional structure of the CEN DNAs. Altogether these data provide further evidence for a model for budding yeast centromeres in which CEN DNA structure could be important for the assembly, activity and/or regulation of the centromere protein-DNA complex.

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