cDNA fingerprinting of osteoprogenitor cells to isolate differentiation stage-specific genes | Zendy

G. Antonio Candeliere | Zendy

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cDNA fingerprinting of osteoprogenitor cells to isolate differentiation stage-specific genes

Author(s) -

G. Antonio Candeliere

Publication year - 1999

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/27.4.1079

Subject(s) - biology , complementary dna , expressed sequence tag , gene , genetics , cdna library , functional genomics , transcriptome , differential display , phenotype , gene expression profiling , computational biology , cellular differentiation , gene expression , genomics , genome

A cDNA fingerprinting strategy was developed to identify genes based on their differential expression pattern during osteoblast development. Preliminary biological and molecular staging of cDNA pools prepared by global amplification PCR allowed discrim-inating choices to be made in selection of expressed sequence tags (ESTs) to be isolated. Sequencing of selected ESTs confirmed that both known and novel genes can be isolated from any developmental stage of interest, e.g. from primitive progenitors, intermediate precursors or mature osteoblasts. EST expression provides insight into possible interrelated physiological functions and putative interacting molecules during differentiation. This method offers a functional genomics approach to isolate differentiation stage-specific genes in samples as small as a single cell.

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