Systematic comparison of gene expression through analysis of cDNA fragments within or near to the protein-coding region | Zendy

Yao Ke | Zendy; Chunxia Jing | Zendy; Paul H. Smith | Zendy; Christopher S. Foster | Zendy; Philip S. Rudland | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Systematic comparison of gene expression through analysis of cDNA fragments within or near to the protein-coding region

Author(s) -

Yao Ke,

Chunxia Jing,

Paul H. Smith,

Christopher S. Foster,

Philip S. Rudland

Publication year - 1999

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/27.3.912

Subject(s) - biology , complementary dna , gene , genetics , coding region , gene expression , restriction enzyme , rapid amplification of cdna ends , microbiology and biotechnology , computational biology , molecular cloning

Life is controlled by the timely and ordered expression of genes. Identification of important genes involved in specific physiological and pathological conditions requires efficient methods to analyse differential gene expression. We describe a novel strategy, namely complete comparison of gene expression (CCGE), for a systematic assessment of differentially expressed genes. Using the CCGE method, double-stranded cDNA is digested with two restriction enzymes that cut with different frequencies, the representative cDNA fragments are generated within or near to the protein-coding region. After being flanked by two different types of adapters, and amplified by a nested suppression PCR, the selected cDNA fragments, representing entire cDNA population, can be divided into 256 subsets; amplified and compared in a systematic manner.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research