A method of one-step enzyme labelling of short oligonucleotide probes for filter hybridisation | Zendy

Anna Guerasimova | Zendy; Ivaylo Ivanov | Zendy; Hans Lehrach | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A method of one-step enzyme labelling of short oligonucleotide probes for filter hybridisation

Author(s) -

Anna Guerasimova,

Ivaylo Ivanov,

Hans Lehrach

Publication year - 1999

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/27.2.703

Subject(s) - oligonucleotide , labelling , biology , biotinylation , chemiluminescence , multiplex , enzyme , oligomer restriction , biochemistry , molecular probe , microbiology and biotechnology , chromatography , dna , chemistry , bioinformatics

Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with streptavidin-conjugated enzyme, hybridised and detected with enzyme-triggered chemiluminescence. The detection of hybridisation signal is linear for two orders of magnitude of target dilution. It is shown to be comparable in sensitivity with standard procedures and with radioactive detection. The method is quick, simple and has potential for automation of large-scale oligo-nucleotide hybridisation and multiplex sequencing.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research