Enhancer of RNA polymerase III gene transcription | Zendy

Michael Sturges | Zendy; Magdalena Bartilson | Zendy; Lawrence J. Peck | Zendy

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Enhancer of RNA polymerase III gene transcription

Author(s) -

Michael Sturges,

Magdalena Bartilson,

Lawrence J. Peck

Publication year - 1999

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/27.2.690

Subject(s) - biology , rna polymerase ii , transcription (linguistics) , enhancer , microbiology and biotechnology , transcription factor ii b , transcription factor ii d , rna polymerase iii , enhancer rnas , transcription factor ii f , xenopus , general transcription factor , rna , gene expression , gene , rna polymerase , promoter , genetics , linguistics , philosophy

A protein responsible for enhanced transcription by RNA polymerase III was identified in extracts from Xenopus oocytes. This protein, called EP3, interacts with a specific DNA sequence adjacent to the 3'-end of a Xenopus somatic 5S RNA gene and forms a distinct band shift complex with a unique DNase I footprint. Enhanced transcription was observed from both 5S RNA and tRNA reporter genes when EP3 binding sites were inserted at different locations and orientations. Removal of the EP3 protein from an oocyte extract abolished this enhanced transcription. In addition, EP3 was shown to stimulate transcription by increasing the rate of transcription complex assembly. EP3 directly discriminates between the somatic and oocyte 5S RNA gene families and may play a significant role in their differential expression during early Xenopus development.

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