A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells | Zendy

Christoph Piechaczek | Zendy; Christiane Fetzer | Zendy; Armin Baiker | Zendy; Juergen Bode | Zendy; Hans J. Lipps | Zendy

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A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells

Author(s) -

Christoph Piechaczek,

Christiane Fetzer,

Armin Baiker,

Juergen Bode,

Hans J. Lipps

Publication year - 1999

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/27.2.426

Subject(s) - biology , vector (molecular biology) , plasmid , shuttle vector , origin of replication , microbiology and biotechnology , scaffold/matrix attachment region , genetics , escherichia coli , chinese hamster ovary cell , gene , coding region , computational biology , cell culture , gene expression , recombinant dna , chromatin remodeling

We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector replicates episomally in CHO cells. It occurs in a very low copy number in the cells and is stably maintained over more than 100 generations without selection pressure.

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