Open AccessA neutralizing antibody against human DNA polymerase epsilon inhibits cellular but not SV40 DNA replication
Author(s) -
Helmut Pospiech,
Inari Kursula,
Waleed AbdelAziz,
Linda H. Malkas,
Lahja Uitto,
Maaret Kastelli,
Maija VihinenRanta,
Sinikka Eskelinen,
Juhani E. Syväoja
Publication year - 1999
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/27.19.3799
Subject(s) - dna polymerase , dna polymerase ii , biology , dna polymerase delta , dna clamp , microbiology and biotechnology , dna replication , polymerase , dna polymerase i , dna synthesis , proliferating cell nuclear antigen , dna polymerase mu , dna , circular bacterial chromosome , reverse transcriptase , polymerase chain reaction , biochemistry , gene
The contribution of human DNA polymerase epsilon to nuclear DNA replication was studied. Antibody K18 that specifically inhibits DNA polymerase activity of human DNA polymerase epsilon in vitro significantly inhibits DNA synthesis both when microinjected into nuclei of exponentially growing human fibroblasts and in isolated HeLa cell nuclei. The capability of this neutralizing antibody to inhibit DNA synthesis in cells is comparable to that of monoclonal antibody SJK-132-20 against DNA polymerase alpha. Contrary to the antibody against DNA polymerase alpha, antibody K18 against DNA polymerase epsilon did not inhibit SV40 DNA replication in vitro. These results indicate that DNA polymerase epsilon plays a role in replicative DNA synthesis in proliferating human cells like DNA polymerase alpha, and that this role for DNA polymerase epsilon cannot be modeled by SV40 DNA replication.
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