Open AccessRegulation of average length of complex PCR product
Author(s) -
Dmitry A. Shagin,
Konstantin A. Lukyanov,
Laura L. Vagner,
Mikhail V. Matz
Publication year - 1999
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/27.18.e23-i
Subject(s) - biology , preference , product (mathematics) , sample (material) , dna , range (aeronautics) , genetics , computational biology , polymerase chain reaction , biological system , statistics , gene , chromatography , mathematics , composite material , materials science , chemistry , geometry
A method to achieve the preference towards longer products during PCR is described. The extent of this preference can be adjusted by slight variation of the PCR conditions. Being combined with the natural tendency of PCR to amplify shorter fragments more efficiently than longer ones, it allows one to regulate the average length of the complex PCR product over a very wide range to make it most suitable for further manipulations. The technique can be used for amplifying any complex DNA sample.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom