Optimization of alternate-strand triple helix formation at the 5'-TpA-3' and 5'-ApT-3' junctions | Zendy

B. Priscille | Zendy; JianSheng Sun | Zendy; JeanFrançois Mouscadet | Zendy; Christian Auclair | Zendy

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Optimization of alternate-strand triple helix formation at the 5'-TpA-3' and 5'-ApT-3' junctions

Author(s) -

B. Priscille,

JianSheng Sun,

JeanFrançois Mouscadet,

Christian Auclair

Publication year - 1999

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/27.15.3029

Subject(s) - triple helix , biology , oligonucleotide , helix (gastropod) , biophysics , nucleotide , sequence (biology) , triple junction , biochemistry , dna , materials science , genetics , gene , optoelectronics , ecology , snail

Alternate-strand triple helix formation was optimized at the two junction steps, the 5"-TpA-3" and 5"-ApT-3" junctions. Footprint experiments, gel retardation assays and thermal denaturation measures on a sequence appropriately designed with two adjacent alternate-strand polypurine tracts points out that the addition of an adenine residue and the removal of one nucleotide should facilitate the crossing strands at the 5"-TpA-3" junction and at the 5"-ApT-3" junction, respectively. These results provide a 'switch code' for the construction of alternate-strand triple helix forming oligonucleotides which open new possibilities for extending the range of applications of antigene strategy.

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