Gel electrophoretic detection of 7,8-dihydro-8-oxoguanine and 7, 8- dihydro-8-oxoadenine via oxidation by Ir (IV) | Zendy

James G. Muller | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Gel electrophoretic detection of 7,8-dihydro-8-oxoguanine and 7, 8- dihydro-8-oxoadenine via oxidation by Ir (IV)

Author(s) -

James G. Muller

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.9.2247

Subject(s) - biology , electrophoresis , microbiology and biotechnology , biochemistry , genetics

Two gel electrophoretic methods are described for detection of 7, 8-dihydro-8-oxoguanine and 7,8-dihydro-8-oxoadenine based on their further oxidation with one-electron oxidants including IrCl62-and IrBr62-. The products of nucleobase oxidation lead to enhanced piperidine-sensitive cleavage and to highly visible stop points in a primer extension assay. 8-oxoG and 8-oxoA lesions may be distinguished by the latter's inability to be oxidized by IrBr62-compared to IrCl62-Comparison is also made to oxidation by MnO4-.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research