Transverse formamide gradients as a simple and easy way to optimise DNA single-strand conformation polymorphism analysis | Zendy

B Paccoud | Zendy

Open Access

Transverse formamide gradients as a simple and easy way to optimise DNA single-strand conformation polymorphism analysis

Author(s) -

B Paccoud

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.9.2245

Subject(s) - single strand conformation polymorphism , biology , dna , formamide , genetics , exon , dna sequencing , computational biology , single strand , gene , microbiology and biotechnology

Although widely used, the detection of DNA mutations by the single-strand conformation polymorphism (SSCP) method is often hampered by the need to examine a large set of electrophoretic conditions in order to select the one suited to the DNA sequence under study. We show here that the use of transverse chemical gradient gels allows for a quick and easy optimisation of SSCP analysis, as exemplified on two mutations in exon 2 of the alpha-1-antitrypsin gene.

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