The importance of base pairing in the penultimate stem of Escherichia coli 16S rRNA for ribosomal subunit association | Zendy

Matthew A. Firpo | Zendy

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The importance of base pairing in the penultimate stem of Escherichia coli 16S rRNA for ribosomal subunit association

Author(s) -

Matthew A. Firpo

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.9.2156

Subject(s) - biology , ribosomal rna , escherichia coli , protein subunit , genetics , base pair , 16s ribosomal rna , ribosomal dna , ribosomal protein , pairing , microbiology and biotechnology , ribosome , bacteria , phylogenetics , gene , rna , superconductivity , physics , quantum mechanics

The influence of base pairing in the penultimate stem of Escherichia coli 16S rRNA (defined as nt 1409-1491) on ribosome function has been addressed by the construction of mutations in this region of rRNA. Two sets of mutations were made on either side of a structurally conserved region in the penultimate stem that disrupted base pairing, while a third set of mutations replaced the wild-type sequence with other base pair combinations. The effects of these mutations were analyzed in vivo and in vitro . The mutations that disrupted base pairing caused significant increases in cell doubling times as well as a severe subunit association defect and a modest increase in frame shifting and stop codon read-through. Restoration of base pairing restored wild-type growth rates, decoding and subunit association, indicating that base pairing in this region is essential for proper ribosome function.

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