Open AccessIntramolecular derivatization of 2'-amino-pyrimidine modified RNA with functional groups that is compatible with re-amplification
Author(s) -
Marian J. Kujau,
Stefan Wölfl
Publication year - 1998
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/26.7.1851
Subject(s) - biology , derivatization , pyrimidine , intramolecular force , rna , genetics , microbiology and biotechnology , biochemistry , gene , stereochemistry , chromatography , high performance liquid chromatography , chemistry
To expand the scope of nucleic acid aptamers as a tool for precise molecular recognition, functional groups that are not naturally present in nucleic acid molecules are desired. For in vitro selection these new functional groups must be compatible with the selection process. The present method allows the introduction of succinimide activated side chains at internal amino groups of 2'-amino-pyrimidine-RNA in a combinatorial fashion that is compatible with enzymatic re-amplification.