Functional complementation by electroporation of human BACs into mammalian fibroblast cells | Zendy

James Hejna | Zendy

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Functional complementation by electroporation of human BACs into mammalian fibroblast cells

Author(s) -

James Hejna

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.4.1124

Subject(s) - biology , electroporation , selectable marker , complementation , microbiology and biotechnology , fluorescence in situ hybridization , genetics , dna , bacterial artificial chromosome , plasmid , phenotype , gene , genome , chromosome

Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.

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