The use of tailed octamer primers for cycle sequencing | Zendy

Sarah Ball | Zendy; Michael A. Reeve | Zendy; Peter Robinson | Zendy; F. Hill | Zendy; D. M. Brown | Zendy; David Loakes | Zendy

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The use of tailed octamer primers for cycle sequencing

Author(s) -

Sarah Ball,

Michael A. Reeve,

Peter Robinson,

F. Hill,

D. M. Brown,

David Loakes

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.22.5225

Subject(s) - histone octamer , biology , primer (cosmetics) , oligonucleotide , dna sequencing , genetics , base sequence , base pair , microbiology and biotechnology , computational biology , dna , histone , nucleosome , chemistry , organic chemistry

Studies have been carried out on the use of octamer oligonucleotides tailed with different base analogues as primers in cycle sequencing reactions. 5-Nitroindole tails improved the performance as primers of a number of octamers. A tail length of three or four 5-nitroindole residues significantly increased the sequencing signal intensity for almost all primers. The use of incomplete libraries of tailed octamer primers for primer walking is discussed.

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