Decreased expression of topoisomerase II in poly(ADP-ribose) polymerase-deficient cells | Zendy

Yvan Canitrot | Zendy; B. Salles | Zendy; Gilbert de Murcia | Zendy

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Decreased expression of topoisomerase II in poly(ADP-ribose) polymerase-deficient cells

Author(s) -

Yvan Canitrot,

B. Salles,

Gilbert de Murcia

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.22.5134

Subject(s) - biology , poly adp ribose polymerase , topoisomerase , microbiology and biotechnology , polymerase , dna repair , gene expression , cell culture , dna , gene , genetics

Recent studies with poly(ADP-ribose) polymerase (PARP)-deficient mice have highlighted the role of this enzyme in genomic stability and response to various genomic insults. In the absence of DNA damaging treatment, we report here that a PARP-deficient cell line (PARP-/-) established from knockout mice displays a decrease in topoisomerase II (topo II) activity as measured by decatenation of kinetoplast DNA. Immunoblotting of whole and nuclear cell extracts showed that reduced activity was associated with decreased amount of the 180 kDa topo IIbeta protein but not of the 170 kDa topo IIalpha. The decreased topo IIbeta expression did not stem from transcriptional regulation of gene expression since levels of topo IIbeta mRNA were similar in PARP (-/-) compared with the parental PARP (+/+) cells. The decreased topo II activity was associated with cell resistance to VP16, a topo II inhibitor. These observations indicate that PARP may play a role in the stabilization and/or distribution of topo IIbeta.

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