Human asparaginyl-tRNA synthetase: molecular cloning and the inference of the evolutionary history of Asx-tRNA synthetase family | Zendy

Kiyotaka Shiba | Zendy; Hiromi Motegi | Zendy; Mitsutaka Yoshida | Zendy; Tomoko Noda | Zendy

Open Access

Human asparaginyl-tRNA synthetase: molecular cloning and the inference of the evolutionary history of Asx-tRNA synthetase family

Author(s) -

Kiyotaka Shiba,

Hiromi Motegi,

Mitsutaka Yoshida,

Tomoko Noda

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.22.5045

Subject(s) - biology , transfer rna , aminoacyl trna synthetase , genetics , complementary dna , peptide sequence , enzyme , amino acid , cloning (programming) , amino acyl trna synthetases , biochemistry , conserved sequence , gene , rna , computer science , programming language

We have cloned and sequenced a cDNA encoding human cytoplasmic asparaginyl-tRNA synthetase (AsnRS). The N-terminal appended domain of 112 amino acid represents the signature sequence for the eukaryotic AsnRS and is absent from archaebacterial or eubacterial enzymes. The canonical ortholog for AsnRS is absent from most archaebacterial and some eubacterial genomes, indicating that in those organisms, formation of asparaginyl-tRNA is independent of the enzyme. The high degree of sequence conservation among asparaginyl- and aspartyl-tRNA synthetases (AsxRS) made it possible to infer the evolutionary paths of the two enzymes. The data show the neighbor relationship between AsnRS and eubacterial aspartyl-tRNA synthetase, and support the occurrence of AsnRS early in the course of evolution, which is in contrast to the proposed late occurrence of glutaminyl-tRNA synthetase.

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