Microinjection of Cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes | Zendy

Thijs de Wit | Zendy; Dubravka Drabek | Zendy; Frank Grosveld | Zendy

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Microinjection of Cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes

Author(s) -

Thijs de Wit,

Dubravka Drabek,

Frank Grosveld

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.2.676

Subject(s) - biology , microinjection , cre recombinase , flp frt recombination , transgene , site specific recombination , recombinase , recombination , microbiology and biotechnology , genetics , rna , cre lox recombination , genetically modified mouse , cytoplasm , ectopic recombination , gene , genetic recombination

We have developed a strategy for producing single copy transgenic mouse lines using Cre-loxP site specific recombination. The method is based on transient expression of the recombinase after injection of in vitro transcribed mRNA into the cytoplasm of fertilised eggs containing multiple copies of the transgene. The success rate of the recombination event is 100% (15 out of 15).

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