End effects in DNA recognition by zinc finger arrays | Zendy

Yen Choo | Zendy

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End effects in DNA recognition by zinc finger arrays

Author(s) -

Yen Choo

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.2.554

Subject(s) - zinc finger , biology , dna , ring finger domain , nucleic acid , dna binding protein , base pair , tandem repeat , dna binding site , lim domain , zinc finger nuclease , duplex (building) , binding site , hmg box , biophysics , genetics , transcription factor , promoter , gene , genome , gene expression

The paradigmatic DNA binding domain from the transcription factor Zif268 contains three zinc finger modules in tandem repeat. When bound to their cognate DNA site the fingers read out the sequence of one DNA strand by making a linear series of successive base contacts. It is shown that the base-specific protein-DNA contacts made from the ends of the Zif268 three-finger array contribute less to the stability of the intermolecular complex than do structurally equivalent contacts from more central regions of the DNA binding domain. The effect is akin to the end fraying observed in duplex nucleic acid molecules.

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