Simple miniaturized gel system for DNA sequence analysis | Zendy

A. Stein | Zendy; Spencer A. Hill | Zendy; Zhao Cheng | Zendy; Minou Bina | Zendy

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Simple miniaturized gel system for DNA sequence analysis

Author(s) -

A. Stein,

Spencer A. Hill,

Zhao Cheng,

Minou Bina

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.2.452

Subject(s) - biology , agarose , dna , dna sequencing , miniaturization , chromatography , gel electrophoresis , polyacrylamide , microscope , dna nanoball sequencing , microbiology and biotechnology , computational biology , base sequence , materials science , nanotechnology , genomic library , genetics , optics , chemistry , physics

A simple miniaturized gel system suitable for DNA sequencing is described. Small ultrathin polyacrylamide gels are cast, eight or more at a time, using standard microscope slides. Gels, ready to use, can be stored for approximately 2 weeks. Gels are run horizontally in a standard mini-agarose gel apparatus. Typical run times are 6-8 min. A novel sample loading system permits volumes of standard sequencing reactions as small as 0.1 microl to be analyzed. Sequencing ladders were visualized using 35S-labeled DNA by autoradiography and by colorimetric detection. Band resolution compares favorably with that of large gels. The methods introduced here serve as a step toward the miniaturization of DNA sequencing and are amenable to automated sample loading and detection.

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