Sequence specificity of the hammerhead ribozyme revisited; the NHH rule | Zendy

Anilkumar R. Kore | Zendy

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Sequence specificity of the hammerhead ribozyme revisited; the NHH rule

Author(s) -

Anilkumar R. Kore

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.18.4116

Subject(s) - hammerhead ribozyme , cleavage (geology) , biology , ribozyme , nucleotide , sequence (biology) , nucleic acid sequence , hairpin ribozyme , microbiology and biotechnology , genetics , rna , dna , gene , paleontology , fracture (geology)

The sequence specificity of hammerhead ribozyme cleavage has been re-evaluated with respect to the NUH rule. Contrary to previous reports it was found that substrates with GAC triplets were also cleaved. This was established in three different sequence contexts. The rate of cleavage under single turnover conditions was between 3 and 7% that of cleavage 3' of GUC. Specificity of cleavage of substrates containing a central A in the cleavable triplet can be described as NAH, where N can be any nucleotide and H any nucleotide but G. As cleavage 3' of NCH triplets has recently been described, the NUH rule can be reformulated to NHH.

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