A simple method for sequencing small DNAs by introducing precise overlapping ends into restriction digestion fragments | Zendy

Graham Rena | Zendy; Miles D. Houslay | Zendy

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A simple method for sequencing small DNAs by introducing precise overlapping ends into restriction digestion fragments

Author(s) -

Graham Rena,

Miles D. Houslay

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.16.3867

Subject(s) - biology , contig , restriction digest , restriction fragment , restriction map , restriction site , restriction enzyme , terminal restriction fragment length polymorphism , fragment (logic) , genetics , genomic dna , sequencing by hybridization , computational biology , dna , dna sequencing , sticky and blunt ends , cloning (programming) , simple (philosophy) , genome , restriction fragment length polymorphism , base sequence , gene , algorithm , polymerase chain reaction , computer science , dna sequencer , programming language , philosophy , epistemology

A method has been devised whereby certain complete restriction digestions can have short overlaps of unique sequence incorporated into the fragments during cloning. Thus one can identify when the DNA fragments are contiguous. Here, this technique is used to produce a contig for a 2.5 kb fragment of genomic DNA. This is a simple approach to ordering digestion fragments without necessarily performing restriction mapping. It is envisaged that this technique will be useful for sequencing cDNAs and small genomic fragments.

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