Solid-phase cDNA library construction, a versatile approach | Zendy

Thomas Roeder | Zendy

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Solid-phase cDNA library construction, a versatile approach

Author(s) -

Thomas Roeder

Publication year - 1998

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/26.14.3451

Subject(s) - cdna library , complementary dna , streptavidin , biology , genomic library , computational biology , biotin , base sequence , biochemistry , dna , gene

A rapid and versatile method for cDNA library construction was developed. It is based on conventional cDNA library synthesis including all enzymatic steps usually required, but is performed on a solid support. The cDNA is immobilised via a biotin residue to streptavidin coupled magnetic beads, which allows rapid and easy to perform changes of buffers and enzymes. Therefore, it combines speed (library construction within a single day) with high quality libraries, making it ideally suited for most purposes.

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